Bi-Monthly Journal
This is a bi-monthly updated blog tracking Chase's progress throughout the completion of his project.
Summary of ProgressSince the end of March, I have made some significant progress on my fellows project. In addition to further refining the SOP (which at this point it should be obvious that I will likely update it to some degree pretty regularly until the end of the project), I also have done some in-lab experiments. Mainly, I focused on growing the original bacteria I ordered a few weeks ago. I am trying to test if the bacteria is still viable, in order to determine if I need to order more. Additionally, I am making sure it can be made back into "competent" bacteria, or cells that can take in the plasmids I need them to. There is a specific process to make an E. coli cell competent (which I have researched, discussed with Mr. Witzel, and added to the SOP) but I need to make sure this batch of bacteria can handle that process before I proceed. I have also begun to take very detailed notes of every thing I do in the lab. I currently have been typing them, however Mr. DeMarte and I discussed getting a physical written notebook instead, as that is the most common way researchers take their notes when doing lab work. More on that soon. Videos + Pictures of Lab Work
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